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cd11b-pe-cy7 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd11b-pe-cy7 antibody
    Cd11b Pe Cy7 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+pe-cy7+antibody/anti+cd11b/pm40512736-83-27-30
    Average 90 stars, based on 1 article reviews
    cd11b-pe-cy7 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Western Blot:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Recombinant:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Activation Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Virus:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    DC Protein Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Incubation:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Flow Cytometry:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Expressing:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Confocal Microscopy:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Comparison:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Staining:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    FACS:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Clone Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Purification:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Control:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Selection:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Software:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Blocking Assay:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).

    Isolation:

    Article Title: Poldip2 controls leukocyte infiltration into the ischemic brain by regulating focal adhesion kinase-mediated VCAM-1 induction
    Article Snippet: Following the Live/Dead viability stain, cells were incubated with anti-mouse CD16/CD32 (Fc block; 1:100, eBioscience), CD3 PE-eFluor610 (1:100; eBioscience), CD4 PE (1:100; eBioscience), CD8b APC-eFluor780 (1:100; eBioscience), CD11b PE-Cy7 (1:200; eBioscience), Ly6G Pacific Blue (1:100; BioLegend), CD45 PerCP-Cy5.5 (1:100; eBioscience), CD11c Alexa Fluor 700 (1:50; BioLegend) Ly6C FITC (1:200; eBioscience) in FACS buffer.

    Article Title: Early Activation of Myeloid-Derived Suppressor Cells Participate in Sepsis-Induced Immune Suppression via PD-L1/PD-1 Axis.
    Article Snippet: The following antibodies were used: CD11b PE-Cy7, Ly-6C (Gr-1) PE, Ly-6G FITC, CD4 FITC, CD8a FITC and CD274 (PD-L1) APC which were purchased from eBioscience (San Diego, CA, US), and CD279 (PD-1) PE which was purchased from BD Bioscience (Sparks, MD, US). qPCR Total RNA from about 5 × 105 isolated PMN-MDSCs from BM of each group.

    Article Title: Neonatal NET-Inhibitory Factor improves survival in the cecal ligation and puncture model of polymicrobial sepsis by inhibiting neutrophil extracellular traps
    Article Snippet: Then, samples were incubated with CD45 APC-Cy7 (Biolegend), CD11b PE-Cy7 (EBioscience), Ly6G BV510 (Biolegend), and CD16/CD32 (Fc-block, EBioscience) for 30 minutes at 37 ° C. Thirty minutes later, cells were washed, fixed, and ran on a Beckman Coulter Cytoflex located in the University of Utah Flow Cytometry Core.

    Article Title: Aberrant nuclear lamina contributes to the malignancy of human gliomas.
    Article Snippet: 1 Introduction Ischemic stroke is caused by intravascular thrombus formation obstructing cerebral blood flow and affects millions of people every year.. Despite improved prophylactic and therapeutic interventions, ischemic stroke is still a major cause of death and disability worldwide (1).. Currently, ischemic stroke therapy is aimed at quick restoration of cerebral blood flow.

    Article Title: Paradoxical imbalance between activated lymphocyte protein synthesis capacity and rapid division rate
    Article Snippet: For mouse lymphocyte stains, antibodies were: CD3ε BV786 (clone 145 – 2 C11, BD), CD4BV510 (clone RM4-5, BD), CD5 APC-R700 (clone 53-7.3, BD), CD8α PE-CF594 (clone 53-6.7, BD), CD11b PE-Cy7 (clone M1/70, eBioscience), CD19 APC-Cy7 (eBio1D3, eBioscience), CD25 BV650 (PC61, BD), CD44 BV605 (IM7, BD), CD44 eFl450 (clone IM7, eBioscience), CD45.1 APC (clone A20, eBioscience), CD45.1 eFl450 (clone A20, eBioscience), CD45.2 eFluor450 (clone 104, eBioscience), CD45.2 PE-Cy7 (clone 104, eBioscience), CD69 PerCP-Cy5.5 (clone H1.2F3, Invitrogen), γδ TCR PE (eBioGL3, GL3, eBioscience), Gr1 PE (clone RB6-8C5, BD), NK1.1 FITC (clone PK136, eBioscience), TCRβ 711 (H57-597, BD), and Vβ5.1/Vβ5.2 PE (clone MR9-4, BD).

    Article Title: Modulating the microenvironment during FVIII uptake influences the nature of FVIII-peptides presented by antigen-presenting cells
    Article Snippet: Afterwards, cells were stained for CD3e PerCP-eFluor 710 (clone 17A2, Thermo Fisher Scientific), CD19 FITC (clone 1D3, BD Biosciences), CD11c APC (HL3, BD Biosciences), CD11b PE-Cy7 (clone M1/70, Thermo Fisher Scientific) and HLA-DR BV605 (clone G46-6, BD Biosciences) and subsequently sorted using a BD FACS Aria III (BD Biosciences).

    Article Title: Tubulin-folding cofactor E deficiency promotes vascular dysfunction by increased endoplasmic reticulum stress
    Article Snippet: Afterward, cells were washed and incubated with viability dye and 294 fluorophore-labeled antibodies against surface epitopes of cells: viability dye, eFluor 506, 295 eBioscience; anti-CD45.2 APC-Cy7, eBioscience; CD31, PE, Biolegend; CD11b, PE-Cy7, 296 eBioscience; Ly6G, FITC, Biolegend; Ly6C, Pacific Blue, BD Bioscience; F4/80, APC, 297 Biolegend; CD19 PerCP, Biolegend; CD90.2, APC-Cy7, eBioscience.

    Article Title: Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria.
    Article Snippet: CD11b (PE-Cy7, clone: M1/70), F4/80 (PE-Cy5, clone: BM8), CD11c (Alexa 700, clone: N418), MHCII (APC eFluor-780, clone: M5/114.15.2), DC-SIGN (APCe-fluor 660, clone:MMD3), and Ly6C (efluor 450, clone: HK1.4) were purchased from eBioscience (San Diego, CA, USA).



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